Primary considerations when choosing a microplate reader are throughput and flexibility. M2. Instrument settings can. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. the Molecular Devices SPECTRAmax® 190 and 250. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. wells across each plate • Enables viewing and tracking of every well in every plate • Reveals additional information on cellular. x. 0001 ABS (calculated) • Light source: Halogen lamp, 8V/50W • Wavelength range: 405, 450, 492, 630 nm, 4 more filters optional. The StakMax stacker also allows users to feed plates into the loading area manually. Each plate reader or washer is calibrated, inspected, and lubricated—any potential issues . x. The following detection cartridge is required: • Fluorescence Polarization (FP-FLUO) Detection Cartridge. 005 OD Normal and Rapid modes, 96- and 384-well plates Fluorescence Read Timing Excitation Filter: 485/20 nm Emission Filter: 528/20 nm 96-well read: 89 sec. Max, FlexStation, Gemini, and earlier instruments as well as import and analysis of data files from FLIPR and Analyst instruments. Washer maintenance kit: 2 X T 2. 0025 OD from 0. Quartz Plate found in: Thick Quartz Ground and Polished Plates, Solid Quartz 96 Well Clear Plate with Clear Base, Quartz Parallel Plate Probes for DMA 7. UV absorbance detection. The soil was amended with either 1. Fluorescence Microplate Reader Comparison Chart. Molecular Devices' microplate readers. The same volume of genotyping mixture (2x KASP Master mix plus KASP Assay mix) was added to the each well. 20 µL (96-well plates) or 10 µL (384. 96- and 384-well. 100 ˜L (96-well) or 25 ˜L (384-well) of Loading Buffer was added to each assay well. x. Refurbished. 2 with 1000 cells. 313 reference gain check fail. A standard curve consisting of MCF7 cells seeded from 20 to 20,000 per well in a 96-well plate was linear across the entire range with r 2 = 0. is required for optimum performa nce with standard 96-well and 384-well format microplates for all top-read applications. QC tools and IQOQ services. 0. SUPPORTED PLATES Microplates having 6, 12, 24, 48, 96, and. 5 ng per well. 3. Powered by a new, optional high-speed EMCCD camera and the new Peak Pro 2 software module, the new FLIPR® Penta High-Throughput Cellular Screening System allows you to measure and analyze peaks of human-derived induced pluripotent stem cells (hiPSCs),. Supersedes 1. * Note: Programming the washer with a final aspirate height of 4. VMax . Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. Category 2 and 3 microplate readers use gradient heating as well. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. These plates are compatible with standard fluorescence microplate readers and are offered in a. From the dropdown Menu, select Wellplates. Click Add to create the plate. The EMax Endpoint ELISA Microplate Reader from Molecular Devices (one of the pioneers of microplate detection) provides a simple and durable dedicated endpoint detection method. Two high-energy xenon flash lamps: Reading capabilities. On the FlexStation 3 reader, the entire series of reactions is monitored in real time with simultaneous on-board reagent pipetting and luminescence detection (Figure 1). The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. , Corning, NY) or Greiner. 6, 12, 24, 48, 96, 384-well plates : ANSI/SLAS Light source. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. The reader includes multiple licenses to the SoftMax® Pro software for endpoint and kinetic analysis of microplate. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. Polypropylene deep well plates, round bottom. Read the plate on the SpectraMax M5, M5e, or FlexStation 3 reader using the optimized settings shown above. 1. SpectraMax iD5 Multi-Mode Microplate Reader (Molecular Devices cat. Molecular Devices LLC 96 well plate reader 96 Well Plate Reader, supplied by Molecular Devices LLC, used in various techniques. Compact design Minimum volume and maximum throughput with 96- or 384-well microplate compatibility Powerful data. The SpectraMax® M2 and M2e Systems from Molecular Devices are multi- detection microplate readers with dual- monochromators, dual-mode cuvette ports, and top- and. TNF standard curve. Bowman Dispensers. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. The ELx808 TM Reader seamlessly interfaces with Lonza's WinKQCL TM Endotoxin Detection and Analysis Software. FlexIII. Multimodal plate reader: Yes: 25 mm: Yes—Max. * Note: Programming the washer with a final aspirate height of 4. EMax. Validation made easy -1) Select the correct plate and the wells that you want to measure. Users can choose to process all plates within the magazine or select a specific number. BMG Labtech - THERMOstar. The screen on the left shows the wells selected for Method MI AF488-10 and Method MI AF488-5 for image acquisition from rows A-D of a 96-well plate at. The four standard filters – 405, 450, 492, 620 nm – allow for a wide variety of ELISAs such as PNPP, ABTS, OPD and TMB. FLIPR System with the 540-590 nm BP Emission FLIPR Filter installed (Molecular Devices Corporation) Membrane Potential Assay Kit (Molecular Devices Corporation, R-8034) Clear, flat-bottom, black- or clear-wall 384- or 96-well plates (see consumables list in Appendix A) Clear, polypropylene compound plates (see consumables list in Appendix A) Flexibility to read a variety of plate formats. Users can also perform measurements such as endpoint, multiple wavelength, kinetic, linear and area scans. The device was able to achieve 3σ limits of detection of ca. 5°C at 37°C. These lab workhorses are used in drug discovery. Reconstitution of. None for any other plate (6, 12, 24, 48 well plates) Flex3: Both Top read and Bottom read. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well microplates. x. x. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. Flexible liquid transfer Using an 8- or 16-channel pipettor, the FlexStation 3 Reader offers added assay flexibility over dispenser-based systems by transferring reagents from 96 or 384 distinct wells in a source plate to a read plate, simultaneously. 6-384 (96- and 384-well with pipetting) 96-well microplates. 9000-0756. The 6-well plates were incubated at 37ºC for 9 days before picking. Temperature control (RT+3°C – 42°C) Liquid dispensers with reagent heater and stirrer. \r Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time. xThe SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. The. Instrument settings can. Here, we demonstrate validation of the SpectraMax ® i3x Multi-Mode Microplate Reader for NanoBRET assays. The picked. UT-6550 Microplate Reader is a reliable and robust instrument for a wide. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Two different agonists, acetylcholine and carbachol, were tested. Molecular Devices LLC Applications: Inquire; Plate Formats: 96-, 384- well. Populate the following info under Description. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. Temperature variation ± 0. . $488. Click Create. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. 0200-6263. The VICTOR Nivo. Intuitive graphical user interface makes each assay extremely easy to set. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. Please click on the links below to view the Microplate Reader Comparison Tables. M5. #SpectraMax L Config) Methods. The equation that allows one to calculate absorbance from % transmittance is. UV cuvettes are ideally suited for measurements at 260 nm, 280 nm and in the visible range. Prior to the reading of each plate, the microplate reader was “blanked” with a plate containing 0. Bay Gray powder coated metal back plate, column, and base. 1 – 2. com. )Plate dimensions: X6001, X6004, X6011, X7001, X7007, X7020, X7022, X5101, X6026. In labs where throughput is a top priority, a device that can screen 96- to 1536-well microplates in under one minute is worthwhile. The SpectraMax® i3x Multi-Mode Microplate Reader measures spectral-based . 200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. Compare this item. An entire 96-well plate can be processed in less than six seconds. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. Published Dec 16, 2020 | Updated Dec 16, 2020. 0, or. The procedures in this protocol support the 96-well reaction plate. Including injection time, it takes approximately 68 secs. For measuring the fluorescence or phosphorescence from a 6-, 12-,24-, 48-, or 96-well plate in either the X or Y direction or for luminescence micro densitometry of TLC plates and electrophoresis gels. Two high-energy xenon flash lamps: Reading capabilities. the Bio-Tek PowerWave™ 200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. 0 . Sample by eye within . Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. We would like to show you a description here but the site won’t allow us. Some will only support one well density (e. More Product Information. Evaporation protection (humidity cassette) Te-Cool™ (active temperature regulation 18-42 °C) Integrated lid handling. Inconsistent results: cannot determine whole well confluence – well after well. Bioz Stars score: 86/100, based on 40 PubMed citations. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the. This eliminates the need for standard curves, and 96–well: 3 mP 384–well: 3 mP 1536–well: 6 mP 1 LUMI Glow luminescence detection for different plates 0200-7014POS Glow for 96-well plates 96–well: 1 pM 96–well: 1. Growth medium was then replaced with fatty acid uptake solution. flat-bottom: 50-60 µl. 0 nm increments Dynamic range > 7 decades Cross-talk < 0. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. 0 nm increments Dynamic range > 7 decades Cross-talk < 0. Locate the User Guide on your SoftMax Pro computer for version 6 and version 7. Part of a plate can be selected so that only a selected area of the plate. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. of 3 wells of a 96-well plate, from 1 of 3 independent experiments. SpectraMax iD5 Multi-Mode Microplate Reader (Molecular Devices cat. For biochemical assays, the washer removes unbound material and unreacted reagents. 96-well plate: 0 to 4 Abs, ±2% at 450 nm, 384-well plate: 0 to 3 Abs ±2% at 450 nm: Measurement Type: Endpoint, Kinetic, Spectra, Multipoint, Kinetic Spectra: No. 2% in. 046 to 0. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Select models. Supplier: Molecular Devices. These models can no longer be repaired. With various on-board features and capabilities, the ThermoMax. Gemini XPS Reader scans. No. SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers. • Photometric range: 0. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. 200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. This instrument is ideal for measuring dual luciferase assays in both 96- and 384-well plates, and SoftMax Pro® software simplifies data collection and analysis with preconfigured protocols. #CA961) Methods. 4D-Nucleofector 96-well Shuttle: RATE CONTROLLED. 1. 1 or 10 Tablet PC with USB connection to instrument: Read Speed: 96-well plate: less than 1 minute; 384-well plate: less than 3 minutes: Automation Compatibility: Hardware and software accessible to third-party hardware/software control, including LIMS. Access this by going to. To assess the sensitivity of each microplate reader, a plate was set up with 32 wells containing PBS only. Molecular Devices SpectraMax i3 Multi-Mode Microplate Reader Pred i3x - AV. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. . Quantitative, whole well cell confluence for every well. Molecular Devices’ SpectraMax® Plus 384 Microplate Reader provides fast and reliable full spectral range detection for cuvettes, 96-well and 384-well microplates. One plate received 200 µL of the BCA working reagent, and the other received 200 µL of the Rapid Gold BCA working reagent. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. 96-well plates - Filter plates with 0. Performing molecular and cellular analyses in multiwell microplate formats is an important methodology for high-throughput screening, or for boosting throughput generally. as well as all other Molecular Devices' microplate readers. MCF7 cells treated with. Find our technical support information along with contact details. Linear, Orbital Slow, and Orbital Fast. 2/24/2015 Molecular Devices FlexStation 3 Russ Desnoyer Our lab needed a way to measure Calcium flux downstream of the human AT1R receptor. 1 - 32 of 54 Sort View Show > Well Plate Reader Accessory PerkinElmer For measuring the fluorescence or phosphorescence from a 6-, 12-,24-, 48-, or 96-well plate in either the X or Y direction or for luminescence micro. This robust system is capable of reading 96 wells, each with its own sample beam and reference detector. These plasticware can come in a variety of formats, such as skirted, semi-skirted, unskirted, clear, colored, and barcoded. SoftMax Pro GxP Software version 5. Molecular Devices (UK) Ltd. The absorbance was then read on the SpectraMax microplate reader at 426 nm, and the resulting OD values for all wells were averaged together to obtain a value of 0. QuickSync. #E010) Pyroplate 96-well microplate (ACC cat. A polyclonal antibody specific to mouse/rat IL-22 was pre-coated onto a 96-well microplate. Quartz 96 Well Plates | 96 Well Plate Flat Bottom | 96 Well Plate Reader | 96 Well Glass. The VMax Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm-750nm), with 4 filter choices included at time of purchase. VIS absorbance detection. Automated live cell imaging – cell counting and confluence. SpectraTest FL1 Fluorescence Validation Plate User Guide Molecular DevicesData are shown as the mean ± s. A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among others. However, for greater flexibility, some instruments can accommodate 6- to 1536-well plate formats. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. • 0. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. 1c shows a cover designed to perfuse 8 wells in series on a standard 96-well microplate, as well as a cover for the entire standard 96-well microplate. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. The list is a standard grouping supplied by Molecular Devices and includes some which cannot be used on this instrument; they can be deleted. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. #3603) SpectraMax ABS Plus Microplate Reader (Molecular Devices cat. EIGHT-CHANNEL OPTICS Plate reads as fast as five seconds MICROPLATES OR CUVETTES Use a 96- or 384-well microplate or a standard cuvette The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. The EMax® Endpoint ELISA Microplate Reader from Molecular Devices, one of the pioneers in microplate detection, offers a ruggedly simple approach to dedicated endpoint assays. 4: Berthold Technologies: TriStar LB 941: Multimodal plate reader: Yes:. Plates were read in the SpectraMax M5 microplate reader at 485ex/515em with bottom read. Explore the best practices for running absorbance-based assays how Molecular Devices absorbance readers can be utilized to optimize said absorbance-based assays. Upgradeable modules are also available including western blot, cell imaging, and fast kinetics with injectors. Selects either 96-well or 384-well plate for a stand-alone read. x. View Product. A Costar® 24-well tissue culture plate with col1 = blanks and doubling cell. The color touch screen provides a visual user interface, making programming fast and intuitive. Top or bottom detection is available with a simple click of a button. Microplate optimization and read height optimization were performed for best results. Microplate Reader. absorbance microplate reader GEA. This can increase throughput and efficiency, thus saving time and space in the lab. 96- and 384-well. High-throughput screening (HTS) can efficiently assay multiple discrete biological reactions using multi-well microplates. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. • High antibody-binding surface improves the overall performance in. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. Back plate and…. Both 96- and 384-well assay formats yielded similar linearity and dynamic range, indicating the suitability of the dual-luciferase assay and SpectraMax i3x system for both formats. The variations include adjustable. 150 µL of deionized water was pipetted into each well of a clean 96-well plate. Expand Microplates and select the newly created plate. Temperature control range from Ambient +4º to 50ºC. #R8340) SpectraMax i3x Multi-Mode Microplate Reader (Molecular Devices cat. x. 000 OD ± 1. The microplate (containing standards) was incubated at 37ºC in the preheated SpectraMax ABS Plus reader for 10 minutes. SpectraMax iD3 reader with touchscreen and inserted USB flash drive. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. The Fluorometric Imaging Plate Reader (FLIPR®; Molecular Devices, Sunnyvale, CA) has made a significant contribution to drug discovery programs in the pharmaceutical industry since the first commercial instruments were introduced 9 yr ago. Click on the crosshair and move it so that it is centered over the well image. All readings were taken in clear, polystyrene 96-shallow-well, flat-bottom plates. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. Published Dec 16, 2020 | Updated Nov 07, 2022. Optimized for fluorescence microplate readers. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. VIS absorbance detection. Below is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. SoftMax Pro GxP Software version 5. • Easy and gentle immobilization of biotin. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Read the microplate • Make sure the purple plate adapter (if required for your plate reader) is in the microplate reader drawer. Powerful, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. (96- and 384-well with pipetting) 6-384 (96- and 384-well). Cross-talk <0. The CellTiter-Glo assay was used to measure ATP. NanoQuant plate. It also integrates with the powerful SoftMax Pro Software for. 1. 340 PC384. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. Molecular Devices has provided scientists with . to each well. VMax . In addition, the iD5 reader measures TRF and FP and. explore more. Range of 96, 384-well SBS microplates . Published Dec 16, 2020 | Updated Dec 06, 2021. Determine concentration using the Beer-Lambert LawPage 1 ™ VERSAmax Tunable Microplate Reader Operator’s Manual for Instruments with Serial Numbers Beginning with BN Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0084 Rev. A Costar® 24-well tissue culture plate with col1 = blanks and doubling cell concentration left to right starting at Col. x. The approximate dead volumes are: 96-well plates: V-bottom: 20-30 µl U-bottom: 30-40 µl flat-bottom: 50-60 µl 384-well plates: approximately 20 µl. Mix well by trituration or plate shaker and incubate for 2 to 5 minutes at room temperature, protected from light. 1% in white 96- and < 0. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. An industry-renowned instrument for monitoring GPCRs and ion channels. No. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. minutes and begin reading microplates with seven mouse clicks. Part Number. and the A1 corner of the plate adapter (Figure 1-2). 030 fmol Fluorescence Polarization (FP). The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. #E010) Pyroplate 96-well microplate (ACC cat. Step 3: Click “Settings” and a screen will appear (Figure 1). x. Making it possible to perform kinetic analysis of solid. 1. Plate formats 6, 12, 24, 48, 96, 384 wells Light source Xenon flash lamp (1 joule/flash) Detector Photomultiplier (R-3896). Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. Values are averages from 5 clean plates, each filled with 200 µL water/well. Some instruments can handle only 96-well plates and lower, whereas others can read up to the ultrahigh throughput densities of 1,536-well plates. The optical system of the SpectraMax 190 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm - 850 nm). Standards, controls and blanks were pipetted into the wells and incubated followed by washing with the Molecular Devices MultiWash™+ Microplate Washer. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. Absorbance was then determined at 450 nm by SpectraMax Paradigm microplate reader (Molecular. An example of dual-luciferase assay results. Measurements for every well are automatically tracked over time. 1 fmol luciferase per well. First choose Read Mode ‘FL’ and Read Type ‘Endpoint’, and then select. Spectramax ® Plus microtiter plate reader (Molecular Devices, Sunnyvale, CA) or similar UV-Vis plate reader; SoftMax ® Pro software (Molecular Devices) (optional) UV compatible quartz plate (for example: # R8024; Molecular Devices) or 96-well Costar ® with UV transparent bottom (for example: #3635 ; Corning Inc. The SpectraMax Paradigm Platform was able to quantify the protein down to the low picomolar level using an AlphaLISA method (Figure 1). 1 mL/well of media. It offers extremely low absorbance background, excellent well to well uniformity, and zero percent cross talk. With more than 40 years of plate reader expertise and life science research, we’ve amassed an extensive. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. #i3x) SpectraMax L Microplate Reader (Molecular Devices cat. Service parts for these models are not being produced and are no longer in stock. Molecular Devices L-Max II Luminescence Microplate Reader. HepG2 cells were plated into a 96-well clear-bottom, white-walled plate at 10,000 cells/well and incubated at 37°C overnight. This can increase throughput and efficiency, thus saving time and space in the lab. VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. 5 OD at 492 nm Computer interface USB 2. Kinetic readings were started immediately with a Molecular Devices FlexStation Plate Reader. Top reading gives greater sensitivity for 96 well plates, unless you have cells adhering to the bottom of the well. Resipher 32x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 32x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 32 wells Utilizes Lucid Lab Cloud software for connection to any…. 0% ± 0. The instrument reads any standard cuvette, microcuvette, test tube and 96 well or UV transparent microplate over full 190-1000 nm wavelength range. SpectraMax 250 User. Running both standard spectrophotometer and microplate. Endpoint, kinetic, spectrum. Upon completion of a plate read, data are delivered to your computer in an Excel-compatible format. Absorbance cuvette port. A = Log 10 (I 0 /I). Description: Enter plate name (optional)(2) Wild‑type KRAS inhibits the migration and invasion of pancreatic cancer through the Wnt/β‑catenin pathway Molecular medicine reports January 1, 2023 Hu X, Zhang R, Yao J, Mu B, Zhao C. We have the following plate-reader instrument available for on-site screens and other projects Molecular Devices Spectramax Paradigm for luminescence, fluorescence, and. 96 wells 384 wells 1 pM 1 pM Optimized bottom sensitivity (fluorescein) 96 wells 384 wells 2 pM 2. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. Figure 2. 2. # SMP54-GXP- 10UL) Pyrochrome Assay chromogenic test reagent (ACC cat. 130009WMicroplate Readers. Corning. one 96-well sample plate can be controlled from 15–40°C,. This 96-well assay has been fully validated on the SpectraMax iD5 reader and other Molecular Devices microplate readers with FP detection to ensure reliable results. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well…. Description. 1% in white 96- and < 0. It offers extremely low absorbance background, excellent well to well uniformity, and zero percent cross talk. 300 µL of Detergent Compatible Bradford reagent was added to each well. These instruments deliver high precision and speed when reading 96 Well Microplates for single endpoint measurements and kinetic assay data gathered over time. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). Click SoftMax Pro Software User Guide. Thermo Scientific Pierce Streptavidin Coated Plates are pre-blocked and ready-to-use polystyrene plates for binding biotinylated antibodies or probes for ELISA and other target-specific assays. When the user applies a set temperature of 37°C, the top is heated to 37. They are widely used in research, drug discovery, [1] bioassay validation, quality control and manufacturing processes in the pharmaceutical and. The advanced design of the Sunrise plate reader includes. A: adipocytes; B: fibroblasts; C: no cells (uptake reagent only). Align the two slots on the bottle holder with the two pegs on the rail. M2. VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. The BCA assay plate was read at 562 nm, the original wavelength setting in the protocol, while for the BCA Rapid. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. Top and bottom reads are. 100 fmol 384-well (75 µL) 0. The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. The Varioskan LUX multimode reader has received HTRF™ certification from Cisbio Bioassays and DLReady™ validation from Promega Corporation. Integrated stacker option. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. 7°C (gradient heating design). Plate Background OD, which was used for subsequent tests, was measured as follows. 96- and 384-well microplates. 0 fmol/well FITC 200 µL in 96 wells Bottom-read detection limit. The ThermoMax performs automatic mixing before a reading and between readings to ensure reliable and precise. perfect for kinetic readings involving growth curves at 5 seconds per plate.